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Diffusion-based multi-stream bioluminescent reaction in a microfluidic device
학술지 Chem. Eng. J., 185-186, 321-327(2012)
저자명 Tae-Woo Lee , Han-Sung Kim , Thuan-Hieu Tran , Jaesung Jang , Dae Sung Yoon , Jin-Hwan Kim, Eun-Ki Kim, Yoon-Mo Koo, Sang Woo Lee., Woo-Jin Chang
In this paper, we report the effect of the number and position of the adenosine triphosphate (ATP)
and luciferase streams on the diffusion-based continuous bioluminescent reaction in a microchannel.
The detectable bioluminescent reaction between substrate (ATP) and enzyme (luciferase) was maximized
when the total flow rate of the substrate and the enzyme solution was around 3 and 8 l/min for
the 2- and 3-stream configurations, respectively. Most of the light was generated inside the luciferase
phase, because the diffusion of over 100 times smaller ATP than luciferase into luciferase phase is
a limiting factor of continuous flow bioluminescence reaction in microchannel. The diffusion-based
reaction of luciferase was enhanced by increasing the number of streams from 2 to 3. The bioluminescence
was higher up to 41% when ATP solution was infused as a middle of three streams (L A L
configuration) comparing with the A L A configuration. The optimized flow rates of ATP and luciferase
solutions on a thick poly(dimethylsiloxane) layer were obtained as 0.15 l/min:0.7 l/min:0.15 l/min
for luciferase:ATP:luciferase solutions, respectively. In addition, a diffusion-based simulation model was
developed for the characterization of the bioluminescent reaction in a microchannel. The numerical simulation
using the model was closely matched with the experimental results with 0.01 level significant
correlations in bivariate correlation coefficient analysis. The reaction between ATP and the luciferase
solution in a microfluidic device can be used for a flow-type bioluminescence sensor for rapid detection
of cells.
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