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Highly efficient enzyme immobilization and stabilization within meso-structured onion-like silica for biodiesel production
학술지 Chem. of Mater., 24, 924−929(2012)
저자명 Seung-Hyun Jun, Jinwoo Lee, Byoung Chan Kim, Ji Eun Lee, Jin Joo, Hyunmin Park, Jong Ho Lee, Sang-Mok Lee, Dohoon Lee, Sangyong Kim, Yoon-Mo Koo, Chae Ho Shin, Seung Wook Kim, Taeghwan Hyeon, and Jungbae Kim
Meso-structured onion-like silica (Meso-Onion-S) was synthesized
and used as a host of enzyme immobilization. Meso-Onion-S has a 200.
300 nm sized primary meso-structured onion building unit, and each onion unit
has highly curved mesopores of 10 nm diameter in a multishell structure.
Nanoscale enzyme reactors (NERs) in Meso-Onion-S were prepared via a twostep
process of enzyme adsorption and subsequent enzyme cross-linking, which
effectively prevents the leaching of cross-linked enzyme aggregates from highly
curved mesopores of Meso-Onion-S. As a result, NERs in Meso-Onion-S
significantly improved the enzyme stability as well as the enzyme loading. For
example, NER of lipase (NER-LP) was stable under rigorous shaking for 40 days,
while the control sample of adsorbed LP (ADS-LP) with no enzyme cross-linking
showed a rapid inactivation due to rigorous enzyme leaching under shaking.
Stable NER-LP was successfully employed to produce biodiesels and fatty acid
methyl esters, from the LP-catalyzed transesterification of soybean oil with methanol. Interestingly, the specific activity of NERLP
was 23 and 10 times higher than those of free LP and ADS-LP, respectively, revealing the importance of LP stabilization in
the form of NER-LP in the presence of organic solvents.
이전글 Optimization of lipase-catalyzed synthesis of caffeic acid phenethyl ester in ionic liquids by response surface methodology
다음글 Effect of ionic liquids on enzymatic synthesis of caffeic acid phenethyl ester