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SCI급 논문 기타학술지 내용
제목
Optimization of lipase-catalyzed synthesis of caffeic acid phenethyl ester in ionic liquids by response surface methodology
학술지 Bioprocess and Biosystems Engineering, 36, 799-807 (2013)
저자명 Sung Ho Ha, Tran Van Anh, Yoon-Mo Koo
Abstract
Lipase-catalyzed caffeic acid phenethyl ester (CAPE) synthesis in ionic liquid, 1-ethyl-3-methylimidazolium
bis[(trifluoromethyl)sulfonyl]imide ([Emim] [Tf2N]), was investigated in this study. The effects of several
reaction conditions, including reaction time, reaction temperature, substrate molar ratio of phenethyl alcohol to caffeic
acid (CA), and weight ratio of enzyme to CA, on CAPE yield were examined. In a single parameter study, the highest
CAPE yield in [Emim][Tf2N] was obtained at 70 C with a substrate molar ratio of 30:1 and weight ratio of enzyme to
CA of 15:1. Based on these results, response surface methodology (RSM) with a 3-level-4-factor central composite
rotatable design (CCRD) was adopted to evaluate enzymatic synthesis of CAPE in [Emim][Tf2N]. The four major factors
were reaction time (36.60 h), reaction temperature (65.75 C), substrate molar ratio of phenethyl alcohol to CA
(20:1.40:1), and weight ratio of enzyme to CA (10:1.20:1).
A quadratic equation model was used to analyze the experimental data at a 95 % confidence level (p\0.05). A
maximum conversion yield of 99.8 % was obtained under the optimized reaction conditions [60 h, 73.7 C, substrate
molar ratio of phenethyl alcohol to CA (27.1:1), and weight ratio of enzyme to CA (17.8:1)] established by our statistical
method, whereas the experimental conversion yield was 96.6 ± 2 %
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